You are tasked with engineering a strain of E.coli that expresses the gene for human epidermal growth factor, EGF. As starting materials, you are given a human cDNA library and a plasmid cloning vector. Your cloning vector conatins a MCS (multiple cloning sites) which include EcoRI, BamHI,Bglll, Notl, and Hindll sequences, the LacZ gene, and the gene for kanamycin resistance. The gene is flanked by EcoRI and Hindll sequences. Describe in 4-5 sentences how to make E.coli express the EGF gene. Be sure to include in your plan how you will evaluate the efficiency of your transformation and expression if your gene.

 

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